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KMID : 0378019740170121671
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New Medical Journal
1974 Volume.17 No. 12 p.1671 ~ p.1674
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The Absorption of Ferritin into the Ectodermal Cells in the Early Chick Embryo
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Abstract
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In the early chick embryos, which were corresponding to the stage 4, definitive streak stage by Hamburger and Hamilton (1951), a little amount of the ferritin solution (NBC Co. 100mg/ml) was
injected into the space between the vitelline membrane and the ectodermal layer of the blastodisc by way of micropipette and than the eggs were reincubated in 37 degrees C. for 2 hours.
The pieces of the blastodisc were carefully removed and fixed in the cold 1% osmic acid in phosphate buffer (pH 7.2). After the dehydration the pieces were embedded in epon 812 by the method of Luft
(1961). The thin sections, stained by the method of Reynolds (1963), were examined by electron microscopy.
The plasma membrane of the lining ectodermal cells showed a relatively even surface or free border which might be less active in the absorption and secretion.
A few ferritin particles were rarely observed in the vesicle or cytoplasm near the free border of the plasma membrane of the ectodermal cells.
Consequently it was speculated that the absorption of the ferritin particles into the ectodermal cells
was sluggish when the ferritin solution was injected into the space between the vitelline membrane and the ectodermal cells of the blastodisc in the early chick embryo.
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